Quantum Sensing Reveals Chemotherapy Induced Persistent Oxidative Footprints in Fixed Brain Cancer Cells
Parhi, J.; Ghosh, A.; Saha, S.; Zaveri, D.; Majumder, A.; Tallur, S.; Majumder, A.; Saha, K. · biophysics · 2026-09-07 · 原文
DOI：10.1101/2025.07.27.666994作者：8 位
Glioblastoma (GBM) is a highly aggressive cancer with poor prognosis despite temozolomide(TMZ) treatment. TMZ induces oxidative stress, causing persistent redox alterations. We report the first use of nitrogen-vacancy centers in nanodiamonds as quantum sensors for T1 relaxometry in fixed GBM cells, detecting stable paramagnetic signatures linked to chemotherapy-induced persistent oxidative damage. U87-MG cells treated with TMZ show dose-dependent T1 shortening, consistent with conventional ROS fluorescence assays. TBHP studies confirm that the T1 response reflects accumulated redox adducts. Measurements remain robust across fixation and permeabilization protocols. Since drug-resistant GBM cells preserve antioxidant signatures, unchanged T1 times after treatment may indicate early resistance phenotypes. This fixed-tissue compatible readout could stratify patient response, guide adaptive therapy, and support biopsy-based diagnostics and post-treatment drug analysis in routinely preserved clinical specimens enabling retrospective studies and better outcomes for future personalized oncology decision making pathways.
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1. 人话版
Glioblastoma (GBM) is a highly aggressive cancer with poor prognosis despite temozolomide(TMZ) treatment.
TMZ induces oxidative stress, causing persistent redox alterations.
2. 领域脉络
本文类目：biophysics，属于其所在研究脉络的最新进展。
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4. 证据与数字
We report the first use of nitrogen-vacancy centers in nanodiamonds as quantum sensors for T1 relaxometry in fixed GBM cells, detecting stable paramagnetic signatures linked to chemotherapy-induced persistent oxidative damage.
U87-MG cells treated with TMZ show dose-dependent T1 shortening, consistent with conventional ROS fluorescence assays.
TBHP studies confirm that the T1 response reflects accumulated redox adducts.
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