Morphological and Functional Effects of Cytoskeletal and Ion-Channel Agents on the Protoscolex of Echinococcus granulosus sensu lato
Carabajal, M. P. A.; Fernandez Salom, M. J.; Martinez, L. J.; Di Lullo, D.; Marcial, E. R.; Albarracin, V. H.; Cantiello, H. F. · pharmacology and toxicology · 2026-09-04 · 原文
DOI：10.64898/2026.04.06.716494作者：7 位
Helminthiases remain a major global health burden, and limitations of current anthelmintic therapies highlight the need for new pharmacological targets. In this study, we examined the effects of ion channel and cytoskeletal modulators on bovine lung protoscoleces (PSCs) of Echinococcus granulosus sensu lato. Compounds acting on ion channels (praziquantel, amiloride, and amlodipine) and cytoskeletal components (albendazole and cytochalasin D) were evaluated using a semi-automated motility assay, methylene blue exclusion to assess viability, and scanning electron microscopy (SEM) to characterize structural damage. All compounds produced concentration-dependent reductions in motility. Amlodipine was the most potent inhibitor of motility, whereas praziquantel and cytochalasin D produced pronounced tegumental alterations. Amiloride was the least potent compound at both endpoints and produced the mildest ultrastructural lesions. In all cases, movement was abolished at concentrations well below those required to reduce viability. Once their shared dependence on concentration was accounted for, the association between the two endpoints disappeared, indicating that paralysis and loss of via
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1. 人话版
Helminthiases remain a major global health burden, and limitations of current anthelmintic therapies highlight the need for new pharmacological targets.
In this study, we examined the effects of ion channel and cytoskeletal modulators on bovine lung protoscoleces (PSCs) of Echinococcus granulosus sensu lato.
2. 领域脉络
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3. 机制拆解
Compounds acting on ion channels (praziquantel, amiloride, and amlodipine) and cytoskeletal components (albendazole and cytochalasin D) were evaluated using a semi-automated motility assay, methylene blue exclusion to assess viability, and scanning electron microscopy (SEM) to characterize structural damage.
All compounds produced concentration-dependent reductions in motility.
Amlodipine was the most potent inhibitor of motility, whereas praziquantel and cytochalasin D produced pronounced tegumental alterations.
4. 证据与数字
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