Investigation into the effects of sodium valproate on prepubertal mouse gonads in vivo
Liugaila, A.; Mitchell, R. T.; Gadd, A.; Duffin, K.; Stefansdottir, A. · pharmacology and toxicology · 2026-09-04 · 原文
DOI：10.64898/2026.09.01.748280作者：5 位
Objective Sodium valproate (SV) is a widely used anti-epileptic drug with well-established reproductive and teratogenic effects, yet its impact on the developing prepubertal reproductive system remains poorly understood. This study investigated the effects of prepubertal SV exposure on gonadal development, including folliculogenesis, testicular architecture and steroidogenic gene expression in male and female mice in vivo. Methods CD1 mouse pups received intraperitoneal injections of saline (control), low or high dose SV (50 or 100 mg/kg, respectively) on postnatal days (PND) 6, 8, and 10. On PND17 the animals were culled and gonads dissected. Ovaries and testes were assessed histologically using haematoxylin and eosin staining. Ovarian follicle number, stage and health were quantified. Testicular tubule morphology and key testicular cell numbers (germ, Sertoli, spermatogonial stem cells, and interstitial/Leydig cells) were evaluated using immunofluorescence with automated image analysis. Expression of key steroidogenic genes (CYP11A1, STAR, CYP19A1 in ovaries; INSL3, STAR, CYP11A1 in testes) was measured by RT-qPCR. Results SV exposure did not significantly affect ovarian follicle
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1. 人话版
Objective Sodium valproate (SV) is a widely used anti-epileptic drug with well-established reproductive and teratogenic effects, yet its impact on the developing prepubertal reproductive system remains poorly understood.
This study investigated the effects of prepubertal SV exposure on gonadal development, including folliculogenesis, testicular architecture and steroidogenic gene expression in male and female mice in vivo.
2. 领域脉络
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3. 机制拆解
Ovaries and testes were assessed histologically using haematoxylin and eosin staining.
4. 证据与数字
Methods CD1 mouse pups received intraperitoneal injections of saline (control), low or high dose SV (50 or 100 mg/kg, respectively) on postnatal days (PND) 6, 8, and 10.
On PND17 the animals were culled and gonads dissected.
Expression of key steroidogenic genes (CYP11A1, STAR, CYP19A1 in ovaries; INSL3, STAR, CYP11A1 in testes) was measured by RT-qPCR.
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