Modelling and measuring effects of shear stress in extrusion bioprinting of endothelial- epithelial cell co-cultures
Davern, J. W.; Weekes, A.; Amaya Catano, J.; Meinert, C.; Bray, L.; Klein, T. J. · bioengineering · 2026-09-07 · 原文
DOI：10.64898/2026.09.02.748801作者：6 位
Extrusion-based bioprinting enables the development of tissue-like constructs; however, the impact of printing-associated shear stress on cell viability and function remains a critical consideration. To address this, we developed a comprehensive workflow combining rheological characterization, computational fluid dynamics (CFD) modelling, and experimental validation to predict and assess shear stress effects during bioprinting. The rheological properties of gelatin methacryloyl (GelMA) at 5 % (w/v, 20 {degrees}C) and 10 % (30 {degrees}C) concentrations were modelled, comparing various non-Newtonian regression models. CFD simulations were validated using micro-particle image velocimetry, showing agreement between predicted and measured velocities. The impact of bioprinting-associated shear stress on cell viability was assessed using a co-culture of human umbilical vein endothelial cells and breast epithelial cells. Immediate post-printing analysis revealed increased apoptosis in GelMA 5 % (w/v, 20 {degrees}C), although 10 % (w/v) GelMA demonstrated higher shear stress levels compared to 5 % GelMA. After 1 day of culture in crosslinked hydrogels, apoptosis increased in extrusion pres
讲义
讲义·推断 依据「原文」自动生成的结构化摘要（推断），非原文表述；以原文为准。
1. 人话版
Extrusion-based bioprinting enables the development of tissue-like constructs; however, the impact of printing-associated shear stress on cell viability and function remains a critical consideration.
To address this, we developed a comprehensive workflow combining rheological characterization, computational fluid dynamics (CFD) modelling, and experimental validation to predict and assess shear stress effects during bioprinting.
2. 领域脉络
本文类目：bioengineering，属于其所在研究脉络的最新进展。
3. 机制拆解
CFD simulations were validated using micro-particle image velocimetry, showing agreement between predicted and measured velocities.
The impact of bioprinting-associated shear stress on cell viability was assessed using a co-culture of human umbilical vein endothelial cells and breast epithelial cells.
4. 证据与数字
The rheological properties of gelatin methacryloyl (GelMA) at 5 % (w/v, 20 {degrees}C) and 10 % (30 {degrees}C) concentrations were modelled, comparing various non-Newtonian regression models.
Immediate post-printing analysis revealed increased apoptosis in GelMA 5 % (w/v, 20 {degrees}C), although 10 % (w/v) GelMA demonstrated higher shear stress levels compared to 5 % GelMA.
After 1 day of culture in crosslinked hydrogels, apoptosis increased in extrusion pres
5. 反例与边界
摘要未声明局限与反例——这是需要警惕的信号，精读时先问边界。
6. 跨领域连接与意外收获
思考本文机制能否迁移到你正在跟进的问题。
7. 可复用方法
把本文机制与你手头项目对照，找一个两周内能验证的最小实验。
8. 术语表
精读时把不熟的术语记入此处，作为下次回忆的锚点。