Spatiotemporal pectin remodelling, glycoproteins, and LEA proteins maintain cell wall integrity during desiccation and rehydration in Ramonda serbica
Pantelic, A.; Ilina, T.; Milic, D.; Kutyrieva-Nowak, N.; Lekic, S.; Popovic, L.; Balliau, T.; Vujisic, L.; Leszczuk, A.; Blein-Nicolas, M.; Vidovic, M. · plant biology · 2026-09-06 · 原文
DOI：10.64898/2026.09.03.749093作者：11 位
Vegetative desiccation tolerance requires specialised cell wall (CW) adaptations to withstand severe mechanical stress during dehydration and rehydration. While intracellular protective strategies in resurrection plants, including Ramonda serbica, are well documented, the CW response remains poorly understood. Here, we integrated immunocytochemical profiling, FTIR spectroscopy, quantification of CW-bound phenolics, transcriptomics, and ionically bound CW proteomics across hydrated (HL), desiccated (DL), and rehydrated states (R1-1h, R2-24h, R3-48h). Reversible CW folding was facilitated by condensed arabinogalactan-proteins (AGPs) and extensins, alongside a site-specific balance between pectin methylesterification and demethylesterification. Structural compaction was further reinforced by the accumulation of CW-bound hydroxycinnamates, which persisted through R1 phase. Moreover, basic 7S globulin, miraculin, -galactosidase, and two LEA4 protein family members were strongly accumulated during DL and R1, providing the first evidence of ionically CW-bound LEA proteins. Initial rewatering (R1) triggered a rapid transcriptomic reactivation of pectin-degrading/modifying enzymes, carbohyd
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1. 人话版
Vegetative desiccation tolerance requires specialised cell wall (CW) adaptations to withstand severe mechanical stress during dehydration and rehydration.
While intracellular protective strategies in resurrection plants, including Ramonda serbica, are well documented, the CW response remains poorly understood.
2. 领域脉络
本文类目：plant biology，属于其所在研究脉络的最新进展。
3. 机制拆解
Reversible CW folding was facilitated by condensed arabinogalactan-proteins (AGPs) and extensins, alongside a site-specific balance between pectin methylesterification and demethylesterification.
4. 证据与数字
Here, we integrated immunocytochemical profiling, FTIR spectroscopy, quantification of CW-bound phenolics, transcriptomics, and ionically bound CW proteomics across hydrated (HL), desiccated (DL), and rehydrated states (R1-1h, R2-24h, R3-48h).
Structural compaction was further reinforced by the accumulation of CW-bound hydroxycinnamates, which persisted through R1 phase.
Moreover, basic 7S globulin, miraculin, -galactosidase, and two LEA4 protein family members were strongly accumulated during DL and R1, providing the first evidence of ionically CW-bound LEA proteins.
5. 反例与边界
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6. 跨领域连接与意外收获
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7. 可复用方法
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8. 术语表
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