Primordial cardiomyocytes orchestrate myocardial morphogenesis and vascularization but are dispensable for regeneration
· 2026-09-08 · 原文
The vertebrate heart is composed of heterogeneous cardiomyocyte (CM) populations; however, the roles of distinct CM subpopulations in heart development and repair remain poorly defined. Here, using single-cell RNA sequencing analysis of adult zebrafish heart, we identified a unique CM subpopulation marked by the expression of phlda2 , which is associated with anaerobic metabolism and different from mature CMs, which are enriched for oxidative phosphorylation genes. We demonstrated that phlda2 + cells constituted a primordial CM compartment localized between compact and trabecular muscles. Genetic ablation of phlda2 + CMs during development severely disrupted heart morphogenesis, leading to defective myocardial trabeculation and compaction, and impaired coronary vascularization. Surprisingl
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1. 人话版
The vertebrate heart is composed of heterogeneous cardiomyocyte (CM) populations; however, the roles of distinct CM subpopulations in heart development and repair remain poorly defined.
Here, using single-cell RNA sequencing analysis of adult zebrafish heart, we identified a unique CM subpopulation marked by the expression of phlda2 , which is associated with anaerobic metabolism and different from mature CMs, which are enriched for oxidative phosphorylation genes.
2. 领域脉络
来源板块：板块二 · 顶级期刊。
3. 机制拆解
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4. 证据与数字
We demonstrated that phlda2 + cells constituted a primordial CM compartment localized between compact and trabecular muscles.
Genetic ablation of phlda2 + CMs during development severely disrupted heart morphogenesis, leading to defective myocardial trabeculation and compaction, and impaired coronary vascularization.
5. 反例与边界
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6. 跨领域连接与意外收获
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7. 可复用方法
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8. 术语表
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