Ribosomal RNA methylation by GidB modulates discrimination of mischarged tRNA
· 2026-09-04 · 原文
Despite redundant cellular pathways to minimize translational errors, errors in protein synthesis are common. Pathways and mechanisms to minimize errors are classified as pre-ribosomal or ribosomal. Pre-ribosomal pathways are primarily concerned with the appropriate charging of tRNAs with their cognate amino acids. By contrast, the ribosomal decoding center is considered ‘blind’ to mischarged tRNAs since these have cognate codon•anti-codon pairing. Here, we identified that in mycobacteria, deletion of the 16S ribosomal RNA methyltransferase gidB led to increased ribosomal discrimination of mischarged tRNAs. Discrimination only occurred in mycobacteria enriched from environments or genetic backgrounds with high rates of mistranslation. GidB deletion was necessary, but not sufficient for red
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1. 人话版
Despite redundant cellular pathways to minimize translational errors, errors in protein synthesis are common.
Pathways and mechanisms to minimize errors are classified as pre-ribosomal or ribosomal.
2. 领域脉络
Pre-ribosomal pathways are primarily concerned with the appropriate charging of tRNAs with their cognate amino acids.
3. 机制拆解
By contrast, the ribosomal decoding center is considered ‘blind’ to mischarged tRNAs since these have cognate codon•anti-codon pairing.
4. 证据与数字
Here, we identified that in mycobacteria, deletion of the 16S ribosomal RNA methyltransferase gidB led to increased ribosomal discrimination of mischarged tRNAs.
5. 反例与边界
Discrimination only occurred in mycobacteria enriched from environments or genetic backgrounds with high rates of mistranslation.
GidB deletion was necessary, but not sufficient for red
6. 跨领域连接与意外收获
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7. 可复用方法
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8. 术语表
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